Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
1999-12-29
2008-03-25
Rao, Manjunath (Department: 1647)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007200, C435S006120, C435S069100, C435S069700, C435S254200, C435S320100, C436S501000
Reexamination Certificate
active
07348151
ABSTRACT:
The invention relates to fusion proteins comprising at least three domains, wherea first domain facilitates membrane localization of the fusion protein in a cellular context, anda second domain has or presumably has a ligand-binding function of a nuclear receptor,a third domain has an activity able to activate a signal pathway connected to a Ras protein in a cell, characterized in that when there is a lack of binding or, alternatively, when there is binding of ligand to the second domain the third domain cannot exert its activity to activate a signal pathway connected to a Ras protein in a cell, despite membrane localization. It additionally comprises inter alia cells which comprise such fusion proteins, assay methods using such cells, which are used inter alia for detecting specific interactions between ligand-binding sections of nuclear receptors and ligands, and kits for use in these assays.
REFERENCES:
patent: 5443962 (1995-08-01), Draetta et al.
patent: 5776689 (1998-07-01), Elledge et al.
patent: WO 94 28914 (1994-12-01), None
Tissue Tropism, In, Medical Microbiology, S. Baron, Ed., 4th Edition, 1996.
Mattioni et al., 1994, Methods in Cell Biology, vol. 43, Chapter 16.
McMahon (2001, Methods in Enzymology vol. 332 pp. 401-417.
The Cell: A Molecular Approach, 2nd Edition, 2000, by Geoffrey M. Cooper; figure 13.34.
Thevelein et al., Molecular Microbiology vol. 33 pp. 904-918,Sep. 1999.
Y.C. Broder et al., “The RAS Recruitment System, A Novel Approach to the Study of Protein-Protein Interactions,” Current Biology, GB, Current Science, vol. 8. No. 20, pp. 1121-1124, Oct. 1998, XP000867036.
A. Aronheim et al., “CHP, A Homologue of the GTPASE CDC42HS, Activates the JNK Pathway And is Implicated in Reorganizing the Actin Cytoskeleton,” Current Biology, GB, Current Science, vol. 8, No. 20, pp. 1125-1128. Oct. 1998, XP000867010.
A. Aronheim: “Improved Efficiency Sos Recruitment System: Expression of the Mammalian GAP Reduces Isolation of Ras GTPase False Positives,” Nucleic Acids Research, vol. 25, No. 16, pp. 3373-3374, Aug. 15, 1997, XP002136255.
A. Aronheim et al., “Isolation of An AP-1 Repressor By A Novel Method for Detecting Protein-Protein Interactions.” Molecular and Cellular Biology, US, American Society for Microbiology, Washington, vol. 17, No. 6, pp. 3094-3102, Jun. 1997, XP000867026.
A. Aronheim et al., “Membrane Targeting of The Nucleotide Exchange Factor SOS is Sufficient for Activating the RAS Signaling Pathway,” CELL, US, Cell Press, Cambridge, NA, vol. 78, pp. 949-961, Sep. 23, 1994, XP002037807.
J. Schlessinger: “How Receptor Tyrosine Kinases Activate RAS,” Trends in Biochemical Sciences, vol. 18, No. 8, pp. 273-275, Aug. 1993, XP002136256, Elsevier, Amsterdam, NL.
Sippel Albrecht E.
Zimmermann André
Forster Hansjoerg
Gamett Daniel C
Millen White Zelano & Branigan P.C.
Rao Manjunath
LandOfFree
Method for the cellular high-throughput-detection of nuclear... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for the cellular high-throughput-detection of nuclear..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the cellular high-throughput-detection of nuclear... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3960272