Method for the cell-free synthesis of proteins

Details Method for the cell-free synthesis of proteins Method for the cell-free synthesis of proteins

1991-05-08

1996-11-05

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 681, 435 912, C12P 1934

Patent

active

055716903

ABSTRACT:
A method is disclosed for the reliable production of proteins by amplifying, transcribing and translating the gene encoding the protein in an essentially cell-free system (gene amplification with transcription/translation, GATT). In the case of dihydrofolate reductase (DHFR), over 1 billion copies of DHFR are produced per original fol gene.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis et al.
patent: 4761375 (1988-08-01), Clark et al.
patent: 4800159 (1989-01-01), Mullis et al.
patent: 4886747 (1989-12-01), Derynck et al.
patent: 4945052 (1990-07-01), Hardy et al.
Spirin et al, Science, vol. 242, pp. 1162-1164, (1988).
Wilson et al, Gene, vol. 77, pp. 69-78, (1989).
Saiki et al, Science, vol. 239, pp. 487-491, (1988).
Mackow et al, Proc. Natl. Acad. Sci. USA, vol. 87, pp. 518-522, (1990).
Horton et al, Gene, vol. 77, pp. 61-68, (1989).
Jones et al, BioTechniques, vol. 8, No. 2, pp. 178-183, (1990).
Sarkar et al, BioTechniques, vol. 8, No. 4, pp. 404-407, (1990).
Sarkar et al, Science, vol. 244, pp. 331-334, (1989).
Smith, et al. (1980) Nucleic Acids Research, vol. 8, p. 2255.

Affiliated with

Also associated with

Rating

Method for the cell-free synthesis of proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for the cell-free synthesis of proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the cell-free synthesis of proteins will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2014327