Method for the analytical separation of viruses

Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...

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204601, G01N 2726, G01N 27447

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active

057230315

ABSTRACT:
The method permits the non-destructive analytical detection and/or the quantification of viruses or viral particles (analytes) in a liquid sample matrix containing organic or inorganic minor constituents, in particular protein moieties and/or nucleotides and/or other viruses. To this end the viruses or the viral particles are separated from the protein moieties and/or nucleotides in the sample matrix using capillary electrophoresis and at the same time the electropherogram associated therewith is recorded. The fractions assigned to the analytes can then be identified as virus peaks in the electropherogram by spectroscopic interpretation with the aid of characteristic maxima. For better identification, a reference sample of a known virus or of a known viral particle can be added to the sample matrix.

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Stellan Hjerten et al, "Carrier-Free Zone Electrophoresis, Displacement Electrophoresis and Isoelectric Focusing In a High-Performance Electrophoresis Apparatus" Journal of Chromatography, 403 (1987) *no month available 47-61.
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