Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1993-06-04
1995-08-15
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 28, 435 4, 435962, 435968, 436 93, 436 91, 436 24, 436800, 436825, 426252, 426102, 4263305, 426333, 426331, 426616, C12Q 126, C12Q 128, C12C 100, C12N 100
Patent
active
054418720
ABSTRACT:
A new enzymatic rate method is disclosed for the analysis of Vitamin C using ascorbate oxidase. Total Vitamin C can be measured in a single analysis, in a procedure amenable to automation. Any dehydroascorbic acid (DHAA) in the sample is reduced to ascorbic acid. Then a coupling agent such as o-phenylenediamine (OPDA) is added to the sample, and any nonspecific reactions with, or spectrometric interferences from, other components of the sample are measured, to be blanked out from the final measurement. Then ascorbate oxidase is added, oxidizing ascorbic acid to DHAA; the DHAA then reacts with the OPDA already present from the prior step, and the quinoxaline derivative product may be measured spectrometrically, e.g., by absorbance at 350 nm.
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Board of Supervisors of Louisiana State University and Agricultu
Leary Louise N.
Runnels John H.
Wityshyn Michael G.
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