Method for the affinity isolation of newly synthesized RNA

Details Method for the affinity isolation of newly synthesized RNA Method for the affinity isolation of newly synthesized RNA

2005-11-01

2005-11-01

Goldberg, Jeanine A. (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091100, C536S023100, C536S024300

Reexamination Certificate

active

06960438

ABSTRACT:
Methods are provided for the isolation of newly synthesized mRNA. Such methods involve the incorporation of biotinylated rNTP analogues into cellular mRNA, and separating biotinylated mRNA from unlabeled RNA. The methods provided herein may be used, for example, for gene discovery, drug screens and studies of the regulation of gene expression.

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