Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1991-02-01
1994-10-18
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
4352401, 4352402, 43524023, 43524025, 435808, 435284, 3649242, 382 6, 382 10, 382 25, C12M 100, C12M 300, C12N 506, C12N 500
Patent
active
053567935
ABSTRACT:
The present invention relates to a method of testing the sensitivity of cancer drugs with cancer cells cultured in vitro. Cancer cells are cultured in a collagen gel substrate. A wide variety of human cancer cell types readily proliferate in the collagen gel substrate, however, fibroblast cells proliferate as well. The measurement of the growth of the cancer cells is hindered by the presence of the fibroblast cells. The present invention solves this problem by counting the number of colonies with an image processor which selectively extracts the image signals of cancer cells and their colonies. In a second embodiment, the growth of cancer cells is determined by measuring the volume of colonies with the image signals of cancer cells and their colonies selectively extracted. The results can be obtained effectively within a short period of time.
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Kobayashi Hisayuki
Koezuka Masahiro
Kondo Naohito
Oda Sachiko
Yasutomi Masayuki
Nitta Gelatin Inc.
Williams Jane A.
Wityshyn Michael G.
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