Chemistry: molecular biology and microbiology – Apparatus – Bioreactor
Patent
1991-08-29
1995-07-04
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Apparatus
Bioreactor
435 4, 435 29, 435 30, 435 32, 435287, 435293, 435809, 422 99, C12Q 100, C12Q 124, C12Q 102, C12Q 104
Patent
active
054299446
ABSTRACT:
A method for evaluating the comparative effect of growth-affecting substances, such as antimicrobial drugs or antibiotics, on different cultures of microorganisms is disclosed. The cultures are plated on the surface of a culture medium (12), such as agar, in adjacent at least partially separated tracks, such as tracks formed as Archimedes spirals. The cultures may be different concentrations of the same microbe or a test culture and a reference culture. The growth-affecting substances (22) are placed in contact with the culture medium on which the cultures have been plated preferably in the form of disks containing a powdered growth-affecting substance which dissolves and diffuses differentially into the culture medium to produce zones (24) of inhibition of growth extending radially outward from the disk. A comparison of the distance between the disk and the point of inhibition of the tracks of the different cultures which intersect the disk permits direct measurement of the comparative effects of the growth-affecting substance.
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Wallace et al J. Microbiological Methods 10 (1989) 303-310.
"Laboratory Methods in Antimicrobial Chemotheraphy", by David S. Reeves, Ian Phillips, J. David Williams, and Richard Wise, published by Churchill Livingstone 1978, pp. 8-30.
Spiral Plater Model D User Manual pp. 1-48 (undated).
Spiral System Instruments, Inc.
Williams Jane A.
Wityshyn Michael G.
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