Method for testing microbial interaction with growth affecting s

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435 30, 435 29, 435292, 435839, 435809, C12Q 118, C12Q 124, C12Q 129, C12M 126

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045144958

ABSTRACT:
A method for testing microbial interaction with growth affecting substances. A pattern of a microbe containing solution is applied on an interaction plate in a programmed concentration. A solution of a growth interacting substance is applied on the interaction plate in a programmed potency in a pattern which contacts the microbe containing solution to cause contact of the microbes with the growth interacting substance. The resultant plate is incubated for a time sufficient to produce visible microbial colonies on the interaction plate. The potency of a growth interacting substance is determined at any point of interest on the incubated interaction plate by correlating the position of the point of interest with the programmed volume of the growth interacting substance deposited at that point.

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de Flora, S., "A Spiral Test Applied to Bacterial Mutagenesis Assays", Mutation Research, vol. 82 (Jul. 1981), pp. 213-227.
User Manual: Model 500A Laser Bacteria Colony Counter, Oct. 1981, Spiral System Instruments, Inc., Bethesda Md.
Schalkowsky et al., "Application of the Spiral Plating Method to Bacterial Interaction Tests," Poster Presentation at 3rd International Symposium on Rapid Methods and Automation in Microbiology, May 27, 1981, Washington D.C.

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