Method for testing for bioaccumulation

Data processing: measuring – calibrating – or testing – Measurement system in a specific environment – Biological or biochemical

Statutory Invention Registration

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C702S022000, C436S071000

Statutory Invention Registration

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H0002225

ABSTRACT:
For use in estimating or predicting bioaccumulation of a chemical analyte, even a surfactant, log Powvalues for the analyte may be determined by calculating the log of the ratio of the concentrations of the analyte in n-octanol and in water, equilibrated using a slow-stir method. In this method, samples of the analyte are prepared and stirred in n-octanol and water (or other largely immiscible solvents) at a rate sufficiently low to avoid emulsions over time at a constant temperature. After stirring, the n-octanol layer and the water layer are sampled and the quantity of analyte in each measured.

REFERENCES:
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Patlewicz, G.Y. et al., “A QSAR Model for the Eye Irration of Cationic Surfacants,” XP-002328589, dated Aug. 26, 1999.
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Southworth, George et al., “Bioaccumulation of Potential and Acute Toxicity of Synthetic Fuels effluents in Freshwater Biotal; Azaaranes,” XP-002328591.
Anonymous, “Extrahieren,” Internet article, pp. 1-3, XP-002328592.
Foreign Communication from a related counterpart application dated Jun. 23, 2005, PCT International Search Report in PCT/GB 2005/000364 & Written Opinion of the International Searching Authority in same case transmittal to AA Thornton Jun. 23, 2005
OECD Guideline for the Testing of Chemicals No. 107, “Partition Coefficient (N-Octanol/Water): Shake-Flask Method,” Jul. 27, 1995. Council for the OECD Paris, France.
OECD Guideline for the Testing of Chemicals No. 117, “Partition Coefficient (N-Octanol/Water): HPLC Method,” Mar. 30, 1989. Council for the OECD Paris, France.
E. Braekevelt et al., “Direct Measurement of Octanol-Water Partition Coefficients of Some Environmentally Relevant Brominated Diphenyl Ether Congenrs,” 51 Chemosphere 563-567.
D. Edlebach et al., “Insights into the Measurements of the Octanol-Water Partition Coefficients from Experiments with Acrylate Esters,” 2 Phys. Chem. 1763-1771, 2000.
Lodge, Keith, “Octanol-Water Partition Coefficients of Cyclic C-7 Hydrocarbons and Selected Derivatives,” 44 J. Chem. Eng. Data 1321-1324 (1999).
McWilliams, P., Payne, G., “Bioaccumulation Potential of Surfactants: A Review,” Presented at Chemistry in the Oil Industry VII, Royal Society of Chemistry & EOSCA, Nov. 2001.
Herbert, B., Dorsey, J., “n-Octanol/Water Partition Coefficient Estimation by Micellar Electrokinetic Capillary Chromatography,” Anal. Chem. 1995, 67, 744-749.

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