Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1985-05-03
1988-07-26
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
4352402, 436 63, C12Q 102, C12N 500
Patent
active
047600209
ABSTRACT:
An in vitro testing method for biocompatibility, particularly toxicological effect, of substances is based on the change in the time for closure of a disruption in a confluent cell culture caused by treatment with the substance. The method can be used to replace a substantial number of toxicity tests, including the Draize test for ocular irritancy.
REFERENCES:
Push, "Scientists Eye Draize Test Alternatives" The John Hopkins Center for Alternatives to Animal Testing Newsletter, vol. 3, No. 1, pp. 1-8 (1984).
Dagani, "In-Vitro Methods May Offer Alternatives to Animal Testing" C & EN (Nov. 12, 1984) pp. 25-28.
Jumblatt et al., "A Tissue Culture Model of Ocular Irritancy" ARVO Abstract (1984).
Jumblatt & Neufeld, "Corneal Epithelial Wound Closure: A Tissue Culture Model" ARVO Abstracts, May 1-May 6, (1983).
Ubels et al., "Healing of Experimental Corneal Wounds Treated With Topically Applied Retinoids", Am. J. Opthalmol. 95:353-358 (1983).
Dagani, "Alternative Methods Could Cut Animal Use in Toxicity Tests" C & EN Oct. 31, 1983, pp. 7-13.
Jumblatt & Neufeld "B-Adrenergic and Serotenergic Responsiveness of Rabbit Corneal Epithelial Cells in Culture", Invest. Opthalmol. Vis. Sci. 24:1139-1143 (1983).
Falahee et al. "Eye Irritation Testing: An Assessment of Methods and Guidelines for Testing Materials for Eye Irritancy" EPA-560/11-82 001 Oct. (1981).
Gipson et al. "Effects of Cytochalasins B & D and Colchicine on Migration of the Corneal Epithelium"Invest. Opthalmol. Vis. Sci. 22:633-642 (1982).
Gipson & Anderson "Effect of Lectins on Migration of the Corneal Epithelium" Invest. Opthalmol. Vis. Sci. 19:341-349 (1980).
Thompson et al. "The Effect of An Eye Derived Growth Factor (EDGF) on Corneal Epithelial Regeneration: Exp. Eye Res. 34:191-199 (1982).
Jumblatt et al. "Cholera Toxin Stimulates Adenosine 3'5' Monophosphate Synthesis and Epithelial Wound Closure in the Rabbit Cornea" Invest. Opthalmol. Vis. Sci. 19:1321-1327 (1980).
Jumblatt and Neufeld "Characterrization of Cyclic AMP-mediated Wound Closure of the Rabbit Corneal Epithelium" current Eye Res. 1:189-195 (1981).
Cintron et al. "A Simple Method for the Removal of Rabbit Corneal Epithelium Utilizing n--Heptanol" Opthalmic Res. 11:90-96 (1979).
Jumblatt & Neufeld "A Tissue Culture Model of the Human Corneal Epithelium" (Opthalmic Pharmacology Unit Eye Research Institute & Dept. of Opthalmology Harvard Medical School, Boston, Mass.
Jumblatt Marcia M.
Neufeld Arthur H.
Eye Research Institute of Retina Foundation
Kepplinger Esther M.
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