Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1978-09-28
1980-11-25
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435253, 435 38, C12Q 104
Patent
active
042359643
ABSTRACT:
In accordance with the invention there is provided a method for identifying or testing an anaerobic microorganism wherein an aqueous suspension of a culture of the microorganism having a culture density of about 2.times.10.sup.8 cells/ml, is brought into contact with an aqueous solution containing an oxidation-reduction indicator which substantially irreversibly undergoes a change in color upon being reduced and a biodegradable test substrate compound which, when catabolized by a microorganism, will engender reduction of the indicator, said aqueous suspension containing nutrient in a concentration sufficient to support microorganism culture growth without engendering reduction of the indicator when said suspension contacts said solution, and said suspension also containing a compound which reduces O.sub.2 but does not reduce the indicator.
REFERENCES:
patent: 4129483 (1978-12-01), Bochner
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