Method for synthesizing stable single-stranded CDNA in eukaryote

Details Method for synthesizing stable single-stranded CDNA in eukaryote Method for synthesizing stable single-stranded CDNA in eukaryote

1991-08-30

1995-07-25

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

4352402, 4352542, 43525421, 4353201, 536 252, C12N 119, C12N 510, C12N 1581, C12P 1934

Patent

active

054361419

ABSTRACT:
A method for producing in vivo stable single-stranded DNAs in eucaryotic cells. The DNAs are multicopy single-stranded DNA (msDNA) structures constituted by a RNA and a DNA portion. The group of genes (retrons) producing said coupled RNA and DNA portions of the msDNAs and the gene encoding reverse transcriptase (RT). The transformed eucaryotes harboring these retrons. The new msDNAs which are encoded by the new retrons. The msDNAs can be used as vectors for antisense DNA and for amplification of inserted genes.

REFERENCES:
patent: 5079151 (1992-01-01), Lampson et al.
Dhundale et al., J. Bact., vol. 170, 1988, pp. 5620-5624.
Lampson et al., Science, vol. 243, 1989, pp. 1033-1038.
Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, 1989, pp. 16.15-16.16.

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