Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1997-02-10
1998-05-26
Guzo, David
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4352523, 43525233, 536 231, C12P 2104, C12N 121, C07H 2104
Patent
active
057563110
ABSTRACT:
The invention relates to a method of manufacturing soluble recombinant proteins from bacteria, in which the plasmid contains the entire gene behind a promoter, this plasmid being expressed and the protein obtained being isolated and purified, in which the cell culture is raised to an OD 600 nm of 0.4 to 0.6; the cell culture is cooled over a period of 15 to 40 minutes to 20.degree. to 26.degree. C.; synthesis is induced by addition of 10 to 55 .mu.M of an inductor; and the induction phase is carried out at a temperature of 20.degree. to 26.degree. C. over a period of 3.5 to 6.5 hours.
REFERENCES:
patent: 5639635 (1997-06-01), Joly et al.
E. Amann and J. Brosius, Gene 40, 1985 (pp. 183-190).
P. Pognonec et al., Nucleic Acids Research vol. 19, No. 23 (1991) p. 6650.
C. Schein, Bio/Technology vol. 7, pp. 1141-1149 (Nov. 1989).
T. Atkinson et al., World Biotech Rep., pp. 399-405 (1988). (3), pp. 291-294!.
Copy of the First Official Action on the German Application and English Translation.
An English translation of Applicant's response.
Otto Bernd
Waschutza Gero
Zakaria Hayssam
Fraunhofer-Gesellschaft zur Forderung der ange-wandten Forschung
Guzo David
Sandals William
LandOfFree
Method for synthesizing soluble recombiant proteins from bacteri does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for synthesizing soluble recombiant proteins from bacteri, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for synthesizing soluble recombiant proteins from bacteri will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1958961