Method for synthesizing single-stranded stem-loop DNA's the prod

Details Method for synthesizing single-stranded stem-loop DNA's the prod Method for synthesizing single-stranded stem-loop DNA's the prod

1995-04-28

2000-03-28

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 914, 435 915, 536 221, 536 245, C12Q 168, C12P 1934, C12N 1564, C07H 2104

Patent

active

060430281

ABSTRACT:
A method of synthesis of new and useful single-stranded DNAs which have a stem-loop configuration (ss-slDNA). The method is an in vivo or an in vitro synthesis. Replicating vehicles which produce these ss-slDNAs. The ss-slDNAs are described. Uses for these slDNAs are disclosed. They can be used for introducing random mutations, they lend themselves for replication by a variant of the PCR method. They can also be used for regulating gene function. Other uses are disclosed.

REFERENCES:
patent: 4357421 (1982-11-01), Emtage et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5118801 (1992-06-01), Lizardi et al.
patent: 5162209 (1992-11-01), Scheele
patent: 5190931 (1993-03-01), Inouye
patent: 5208149 (1993-05-01), Inouye
patent: 5215899 (1993-06-01), Dattagupta
patent: 5272065 (1993-12-01), Inouye et al.
patent: 5278051 (1994-01-01), Seeman et al.
patent: 5312728 (1994-05-01), Lizardi et al.
patent: 5470722 (1995-11-01), Jones
Kikuchi, et al., EMBO Journal, 4(7): 1881-1886 (1985).
Land, et al., Nucl. Acid. Res., 9:2251-2266 (1981).
Merchlinsky, J. Virol., 64:3437-3446 (1990).
Nobile, et al., Intervirology, 25:158-171 (1986).
Ohshima, et al., Proc. Natl. Acad. Sci. USA, 89:1016-1020 (1992).
Sambrook, et al., Molecular Cloning, A Laboratory Manual, 1989, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 1.12-1.13, 1.21-1.41, 1.85-1.86, 1.3-1.6.
Tsurimoto, et al., J. Biol. Chem., 266(3): 1950-1960 (1991).

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