Method for synthesizing polynucleotides

Details Method for synthesizing polynucleotides Method for synthesizing polynucleotides

2008-04-15

2010-12-07

Mummert, Stephanie K (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120

Reexamination Certificate

active

07846695

ABSTRACT:
The present invention realized isothermal and rapid polynucleotide synthesis by using as templates polynucleotides having a structure capable of forming loops, and combining a plurality of primers capable of providing a starting point for complementary strand synthesis to such loops. If the LAMP method is applied, all reactions can be carried out isothermally and rapidly since the template polynucleotides themselves can also be synthesized by an isothermal reaction.

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Whitcombe et al., “Detection of PCR Products Using Self-Probing Amplicons and Fluorescence,” Nat. Biotech. 17:804-807 (1999).

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