Method for synthesizing cDNA

Details Method for synthesizing cDNA Method for synthesizing cDNA

2005-09-27

2005-09-27

Sitton, Jehanne (Department: 1637)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S091200, C435S091210, C435S183000

Reexamination Certificate

active

06949634

ABSTRACT:
A method for synthesizing cDNA characterized by performing a reverse transcription reaction in the presence of an enzyme having a reverse transcriptional activity and another enzyme different from the former one which as a 3′-5′ exonuclease activity.

REFERENCES:
patent: 5407800 (1995-04-01), Gelfand et al.
patent: 5618703 (1997-04-01), Gelfand et al.
patent: 5624833 (1997-04-01), Gelfand et al.
patent: 5830714 (1998-11-01), Swaminathan et al.
patent: 6406891 (2002-06-01), Legerski
patent: 6410277 (2002-06-01), Barnes
patent: 0 878 553 (1998-11-01), None
patent: 0 921 196 (1999-06-01), None
patent: 10-136998 (1998-05-01), None
patent: WO 97/39113 (1997-10-01), None
Cline et al. PCR fidelity of Pfu DNA Polymerase and other thermostable DNA Polymerases, Nuclear Acids Res., vol. 24, No. 18, pp. 3546-3551, 1996 Stratagene Catalog. Gene Characterization kits. Stratagene Catalog, p. 39, 1988.
Lu et al, “Construction and quality of complementary DNA libraries prepared from cytoplasmic RNA not enriched in pollyadenine positive DNA”,Biological Abstracts No. 87058715; Gene (1988), vol. 71, No. 1, pp. 157-164.
Mallet et al; “Continuous RT-PCR Using AMV-RT and Taq DNA Polymerase: Characterization and Comparison to Uncoupled Procedures”; Bio Techniques (1995), vol. 18, No. 4, pp. 678-687.
Murakami, Y.; “Protein”, Nucleic Acid and Enzyme (Japanese), 1996, vol. 41, No. 5, pp. 595-602.
Kawakami et al; “Cell Technology” (Japanese); 1997, vol. 16, No. 5, pp. 725-732.
Boehringer Mannheim 1997 Biochemicals Catalog, pp. 146-147, 150-153 and An Instruction Manual from Titan™ RT-PCR System, Cat. No. 1855476.
Catalogue Clonlech, Innovative Tools to Accelerate Discovery, 30:179 (1996).

Affiliated with

Also associated with

Rating

Method for synthesizing cDNA does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for synthesizing cDNA, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for synthesizing cDNA will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3374104