Method for synthesizing an enzyme-catalyzed polymerized monolaye

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process...

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435128, 435156, 435189, 435190, 435191, 435192, 435310, C12P 722, C12P 100, C12N 902, C12N 904

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active

051438283

ABSTRACT:
A method for synthesizing enzyme-catalyzed polymers using the Langmuir-Blodgett technique. In one embodiment, the process comprises spreading one or more enzyme-polymerizable monomers on a water-miscible solvent. The monomers are sufficiently surface active that they align themselves on the air-solvent interface. Next, pressure is applied to the interface to form a monolayer made up of the monomers. An enzyme is then introduced into the solvent, causing polymerization of the monomers in the monolayer. The polymeric monolayers produced by the present method are easier to process and have reduced cross-linking and branching as compared to similar polymers produced in bulk by enzyme-catalyzed reactions.

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