Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2007-04-17
2007-04-17
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
Reexamination Certificate
active
10222368
ABSTRACT:
Synthesis of the second strand of cDNA using pol ∝-primase is provided. Pol ∝-primase is used to initiate primers on a cDNA strand de novo, followed by extension of the primer with pol ∝-primase, a second DNA polymerase or a combination thereof. In a preferred embodiment, pol ∝-primase extends the primer, followed by addition of a second DNA polymerase, preferably Klenow fragment.
REFERENCES:
patent: 5597713 (1997-01-01), Kato et al.
patent: 5962272 (1999-10-01), Chenchik et al.
patent: 6096499 (2000-08-01), Kozlowski et al.
patent: 6132997 (2000-10-01), Shannon
patent: 6143528 (2000-11-01), Hayashizaki
patent: 6326175 (2001-12-01), Guegler et al.
patent: 6582906 (2003-06-01), Cao et al.
patent: 2004/0014072 (2004-01-01), Ishino et al.
Gubler et al. A simple and very efficient method for generating cDNA libraries. Gene, vol. 25, pp. 263-269, 1983.
Harrington et al. Initiation of RNA-primed DNA synthesis in vitro by DNA polymerase alpha-primase. Nucleic Acids Res., vol. 23(6), pp. 1003-1009, 1995.
Holmes, AM et al. Initiation of DNA synthesis by the calf thymus DNA polymerase-primase complex. J Biol Chem., vol. 260, No. 19, pp. 10840-10846, 1985.
Arezi et al. Eukaryotic DNA primase. TIBS, vol. 25, p. 572-576, Nov. 2000.
Hubscher et al. Eukaryotic DNA polymerases, a growing family. TIBS, vol. 25, p. 143-147, Mar. 2000.
Alessio et al. Second-strand cDNA synthesis withE.coliDNA polymerase I and RNase H: the fate ofinformation at the mRNA 5′ terminus and the effect ofE.coliDNA ligase. Nucleci Acids Res., vol. 16, No. 5, pp. 19992014, 1988.
Harrington et al. Initiation of RNA-primed DNA synthesis in vitro by DNA polymerase alpha-primase. Nucleic Acids Res., vol. 23, No. 6, pp. 1003-1009, 1993.
Eberwine J., “Amplifications of mRNA Populations Using a RNA Generated from Immobilized Oligo(dT)-T7 Primed cDNA”,Biotechniques(1996), 20:584-591.
Eberwine J. et al., Analysis of Gene Expression in Single Live Neurons,PNAS USA(1992), 89:3010-3014.
Gronostajski R. et al., “Purification of a Primase Activity Associated with DNA Polymerase α from HeLA Cells”,The Journal of Biological Chemistry(1984), 259: 9479-9486.
Ilsely D. et al., “Acyclic Guanosine Analogs Inhibit DNA Polymerases α, δ, ε With Very Different Potencies and Have Unique Mechanisms of Action”,Biochemistry(1995), 34:2504-2510.
Johnson, K., “Conformational Coupling in DNA Polymerase Fidelity”,Annu. Rev. Biochem(1993), 62:685-713.
Kirk B. et al., “Eucaryotic DNA Primase Does Not Prefer To Synthesize Primers at Pyrimidine Rich DNA Sequences When Nucleoside Triphosphates Are Present at Concentrations Found in Whole Cells”,Biochemistry(1997), 36:6725-6731.
Lodish et al., “Eukaryotic Proteins that Replicate SV40 DNA in Vitro Exhibit Similarities and Differences withE. ColiReplication Proteins”,Molecular Cell Biology, 3rded., p. 378-380.
Phillips J. and Eberwine J., “Antisense RNA Amplification: A Linear Amplification Method for Analyzing the mRNA Population from Single Living Cells” ,Methods: A Companion to Methods in Enzymology, (1996), 10:283-288.
Sambrook et al., Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Press (1989)—Table of Contents.
Stillman, B., “Initiation of Eukaryotic DNA Replication in Vitro”,Ann. Rev. Cell Biol. (1989), 5:197-244.
Thompson H. et al., “Arabinofuranosyl Nucleotides Are Not chain-Terminators during Initiation of New Strands of DNA by DNA Polymerase α-Primase”,Biochemistry(1995), 34 11198-11203.
Van Gelder R. et al., “Amplified RNA Synthesized from Limited Quantities of Heterogeneous cDNA”,PNAS USA(1990), 87:1663-1667.
Waga, S. and Stillman, B., “Anatomy of a DNA Replication Fork Revealed by Reconstitution of SV40 DNA Replication in Vitro”,Nature(1994), 369:207-212.
Agilent Technologie,s Inc.
Horlick Kenneth R.
LandOfFree
Method for synthesis of the second strand of cDNA does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for synthesis of the second strand of cDNA, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for synthesis of the second strand of cDNA will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3755017