Method for synthesis of anhydrothrombin

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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A61K 3848

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059393041

ABSTRACT:
A method for a synthesis of anhydrothrombin is provided which features a short process, an easy procedure, and high yields.
The method comprises

REFERENCES:
"Anhydrotrypsin: New Features in Ligand Interactions Revealed by Affinity Chromatography and Thionine Replacement" by Yokosawa and Ishii; J. Biochem, 81, pp. 647-656 (1977).
"Anhydrotrypsin and Trypsin: Subtle Difference in the Active-site Conformations Detected by Chemical Modification and CD Spectroscopy" by Yokosawa and Ishii; J. Biochem, 81, pp. 657-663 (1977).
"The Preparation of Anhydro-Trypsin and Its Reactivity With Naturally Occurring Proteinase Inhibitors" by Ako, Foster and Ryan, Biochemical and Biophysical Research Communications, vol. 47, No. 6, 1972, pp. 1402-1407.
"The Purification by Affinity Chromatography of a Proteinase Inhibitor Binding Species of Anhydro-Chymotrypsin" by Ako, Ryan and Foster; Biochemical and Biophysical Research Communications, vol. 46, No. 4, 1972, pp. 1638-1645.
"Preparation and Characterization of Anhydrothrombin" by Ashton and Scheraga; Biochemistry 1995, 34, pp. 6454-6463.
"Preparation of Anhydro-thrombin and Its Interacton with Plasma Antithrombin III" by Tomono and Sawada; ACTA Haematologica Japonica, vol. 49, No. 4, pp. 969-979, 1986.
Japanese Patent Application No. JP-B-59-7694 with English Abstract.

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