Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-09-14
1994-03-15
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 28, 435188, 435810, 435962, 436175, 436825, 436826, C12N 996, C12Q 128, G01N 33535
Patent
active
052945359
ABSTRACT:
The invention concerns a method for detecting an analyte in a blood sample or a derived fraction thereof. In this method one uses the binding affinity between the analyte and its specific binding agent. Moreover, a reaction component containing a peroxidase label is added to the sample and specific binding agent to form a reaction mixture. The incubation of the reaction mixture, containing the analyte and its specific binding agent, with the peroxidase-labeled reaction component has to be carried out in the presence of a quaternary ammonium salt or ionic surfactant.
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Heath, R. L. et al., "A New Sensitive Assay for the Measurement of Hydro-perosidase," Analytical Biochem. 76, pp. 184-192 (1976), USA.
Porstmann, B., "Comparison of Chromogens for the Determination of Horseradish Peroxidase as a Marker in Enzyme Immunoassay," J. Clin. Chem. Clin. Biochem., vol. 19, 1981, pp. 435-439, USA.
Voller et al., Enzyme Imunoassays, Chap. 6, Alternative Immunoassays, Ed. W. P. Collins, 1985, pp. 77-86.
Goossens Petrus A. L.
Gribnau Thomas C. J.
Kuypers Leonardus P. C.
Akzo N.V.
Bobrowicz Donna
Gormley Mary E.
Kepplinger Esther L.
Parsons Nancy J.
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