Method for suppressing inhibition of enzyme-mediated reactions b

Details Method for suppressing inhibition of enzyme-mediated reactions b Method for suppressing inhibition of enzyme-mediated reactions b

1997-01-06

1998-12-08

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 6, 435 911, 435 912, 435183, 435188, C12Q 100, C12P 1934

Patent

active

058467017

ABSTRACT:
Methods and kits for the use of a non-ionic detergent to suppress enzyme inhibition in a reaction solution due to the presence of inhibiting ionic detergent. Prior to reaction, the reaction mixture is given an effective amount of a non-ionic detergent, and agitated. The enzyme is then added, and the enzymatic reaction is the allowed to proceed. Also disclosed are preferred embodiments of the present invention, and kits for nucleic acid amplification of a biological sample in the presence of an ionic detergent.

REFERENCES:
patent: 5004684 (1991-04-01), Simpson et al.
patent: 5175094 (1992-12-01), Mallonee
patent: 5358690 (1994-10-01), Guirguis
patent: 5538870 (1996-07-01), Noeth et al.
Gelfand, "Taq DNA Polymerase", in PCR Technology, H. A. Erlich, Ed., Stockton Press, 1989, pp. 17-22.
Bachmann et al., Nucleic Acids Res. 18(5), 1309 (1990).
Loeffelholz et al., J. Clin. Micro. 30(11), 2847-2851 (1992).
Herman, et al. Direct Detection of Chlamydia trachomatis in Urogenital Specimens Using a Rapid, PCR-Based Assay System. Abstract, American Society for Microbiology; Mar. 1991.
Helenius A. and K. Simons. Solubilization of membranes by detergents. Biochim. Biophys. Acta 415:29-79, Elselvier Scientific Publication Co., Amsterdam (1975).
Ey, P.L. and E. Ferber. Calf thymus alkaline phosphatase II; interactions with detergents. Biochim. Biopys. Acta 480:163-177, Elselvier North-Holland Biomedical Press (1977).
Stralfors, P. et al. Removal of non-ionic detergent from proteins fractionated by electrofusing. Biochim. Biophys. Acta 533:90-97. Elsevier North-Holland Biomedical Press (1978).
Saito, M. et al. Action of arthrobacter ureafaciens sialidase on sialglycolipd substrates. J. Biol. Chem. 254(16):7845-7854 (1979).
Berge, R. et al. Variations on the activity of microsomal palmitoyl-CoA hydrolase in mexed micelle solutions of palmitoyl-CoA and non-ionic detergents of the Triton X series. Biochim. Biophys. Acta 666:25-35, Elselvier North-Holland Biomedical Press (1981).
Corti, M. et al. GMI-Ganglioside-Triton X-100 mixed micelles; changes of micellar properties studied by laser-light scattering and enzymatic methods. Chemistry and Physics of Lipids 28:197-214 (1981).
Wittenburg, C. And E. Tripplett. A detergent-activated tryosinase from xenopus laevis. J. Biol. Chem. 260(23):12542-12546 (1985).
Tandon, S. And P. Horowitz. Detergent-assisted refolding of guanindinium chloride denatured rhodanese. J. Biol. Chem. 262(10):4486-4491 (1987).
Baselski, V. et al. Comparison of Roche Chlamydia PCR test to McCoy cell culture in obstetric patients. Dept. Of Pathology and Obstet. Gynecology, Univ. Of Tenn., Memphis, TN. Abstract from meeting of the American Society for Microbiology: May 26-30, 1992, Atlanta, GA.
Package insert for brochure of Amplicor's Chlamydia trachomatis test manufactured by Roche-Diagnostic Systems, Nutley, New Jersey, Jun. 1993.

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