Method for stimulating cell multiplication, differentiation, emb

Details Method for stimulating cell multiplication, differentiation, emb Method for stimulating cell multiplication, differentiation, emb

1990-06-14

1995-04-25

Benzion, Gary

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524046, 43524054, C12N 500, C12N 504

Patent

active

054098289

ABSTRACT:
The invention provides a process for stimulating the multiplication, differentiation or somatic embryogenesis of higher plant cells in tissue culture by the addition to the culture medium an effective amount of extensin preparation derived from higher plant tissue. Also, provided are tissue cultures which have such stimulating amount of extensin present. Finally, provided by the invention is the sterile extensin preparation which has the stimulating activity, preferably the extensin used being pure or substantially pure.

REFERENCES:
patent: 4801545 (1989-01-01), Stuart et al.
Chen et al. (1985) The Embo Journal, vol. 4, No. 9, pp. 2145-2151.
Osima et al. (1978) Fronties of Plant Tissue Culture, Ed. Thorpe, pp. 265-275.
Gamborg et al. (1981) Plant Tissue Culture, Ed. Thorpe, p. 26.

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