Method for sterilizing transformed cells

Details Method for sterilizing transformed cells Method for sterilizing transformed cells

2005-03-22

2005-03-22

Patterson, Jr., Charles L. (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Utilizing media containing lower alkanol

C435S243000

Reexamination Certificate

active

06869791

ABSTRACT:
There is provided a method for sterilizing a transformed microorganism, which is characterized by mixing at a temperature range of 25° C. or higher to less than 35° C. a solution containing a transformed microorganism belonging toEscherichiaintroduced with a DNA coding for an enzyme having a thermal denaturation temperature of 50° C. or higher, with at least one member selected from the group consisting of monovalent alcohols having one to three carbon atoms and acetone, in an amount of 10 to 35% by weight of the solution.

REFERENCES:
patent: 4612169 (1986-09-01), Iwasaki et al.
patent: 4680262 (1987-07-01), Bochner et al.
patent: 4966844 (1990-10-01), Tallen et al.
patent: 5137821 (1992-08-01), Sagai et al.
patent: 0 497 103 (1992-08-01), None
patent: 0 969 094 (2000-01-01), None
patent: 1 057 894 (2000-12-01), None
patent: 59-55186 (1984-03-01), None
patent: 06 030774 (1994-08-01), None
ilngram, L.O., et al. (1980) J. Bacteriol. 144(2), 481-488.*
Bokinbobai No. Kagaku, Published by Sankyo-Shuppan Co. on Jan. 10, 1982.
Nishizawa et al., “Stereoselective Production of (+)-trans-Chrysanthemic Acid by a Microbial Esterase: Cloning, Nucleotide Sequence, and Overexpression of the Esterase Gene ofArthrobacter GlobiformisinEscherichia Coli,” Applied and Environmental Microbiology, vol. 61, No. 9, pp. 3208-3215 (Sep. 1995).
Toida et al., “Cloning and Sequencing of the Triacylglycerol Lipase Gene ofAspergillus Oryzaeand Its Expression inEscherichia Coli,” FEMS Microbiology Letters, 189, pp 159-164 (2000).
Field et al., “Biological Elimination of Polycyclic Aromatic Hydrocarbons in Solvent Extracts of Polluted Soil by the White Rot Fungus,BjerkanderaSP, Strain BOS55,” Environmental Technology, vol. 17, pp. 317-323 (1996).
Ingram, L.O., “Mechanism of Lysis ofEscherichia Coliby Ethanol and Other Chaotropic Agents,” Journal of Bacteriology, vol. 146, No. 1, pp. 331-336 (Apr. 1981).
Database EMBL oNLINE! EBI; Aug. 23, 2000, Toida J. et al.: “Aspergillus OryzaeTgIa Gene For Triacylglycerol Lipase, Complete CDs”; Database Accession No. AB039325, XP002229311.
Anonymous: From Analytical To Preparative/Industrial Mitsubishi Chemical, Online! Dec. 23, 1999, XP002229312, Retrieved from Internet.

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