Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1983-11-09
1987-03-17
Martinell, James
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 68, 435 70, 435 71, 435 91, 435253, 435317, 435849, 536 27, 935 13, 935 29, 935 56, 935 73, 935 83, C12N 1500, C12N 120, C12N 100, C12P 2100, C12P 2102, C12P 2104, C12P 1934, C12R 119, C07H 2104
Patent
active
046507612
ABSTRACT:
The present invention is an improvement in the method for stabilizing and selecting host cells containing recombinant DNA. The method involves transforming a host cell with a recombinant DNA cloning vector which contains the .about.2.5 kb BglII cI repressor gene-containing restriction fragment of bacteriophage .lambda. and then lysogenizing the transformed host cell with a lysogenic organism which contains a marker which is lethal or conditionally lethal in the host cell but which is repressed in the transformed host cell by the repressor gene contained in the recombinant DNA cloning vector. The vector additionally contains a gene which codes for the expression of human proinsulin. The invention further comprises related recombinant DNA cloning vectors, transformed host cells and lysogenized transformed host cells.
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Hershberger Charles L.
Rosteck, Jr. Paul R.
Dahling Gerald V.
Eli Lilly and Company
Martinell James
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