Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-03-04
1995-03-14
Ceperley, Mary E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 6, 435 792, 435 793, 435962, 564 2, G01N 33553
Patent
active
053976991
ABSTRACT:
A method for stabilizing an alkanol amine buffer against substantial degradation so that enzyme activity can be optically determined in such a buffer is described. Stabilization is achieved by adding a stabilizing effective amount of a stabilizing agent to the buffer wherein the stabilizing agent (i) does not inhibit enzyme activity and (ii) does not interfere substantially with the optical determination in the wavelength region of measurement.
REFERENCES:
patent: 3207790 (1965-09-01), Glew et al.
patent: 3535260 (1970-10-01), Singh
patent: 3567779 (1971-03-01), Currier et al.
patent: 3742059 (1973-06-01), Dowd
patent: 4824784 (1989-04-01), Cantarow
patent: 4840777 (1989-06-01), Faucher
patent: 4885240 (1989-12-01), Wu
patent: 4892817 (1990-01-01), Pawlak
patent: 5113017 (1992-05-01), Smith et al.
patent: 5135847 (1992-08-01), Hoke
"Reagent for Analysis of Alkali Phosphatase," Database, WPI Week 7613, Derwent Publications Ltd., AN 76-23250X & JP51016989, Wako Pure Chem Ind KK, Feb. 10, 1976.
Rej, R. and Bretaudiere, J-P, "Effects of Metal Ions on the Measurement of Alkaline Phosphatase Activity," Clinical Chemistry, vol. 26, No. 3, Mar. 1980, pp. 423-428.
Thompson, R. Q. et al., "Zeptomole detection limit for alkaline phosphatase using 4-aminophenylphosphate, amperometric detection, and an optical buffer system," Analytica Chimica Acta, vol. 271, No. 2, Jan. 18, 1993, pp. 223-229.
Krebs, H. A., The Use of `CO.sub.2 Buffers` in Manometric Measurements of Cell Metabolism, J. Biochem, 48, 349-359, 1951.
Davis James E.
Lau Hon-Peng P.
Neelkantan Neel V.
Ceperley Mary E.
E. I. Du Pont de Nemours and Company
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