Method for stabilization of proteins in solution

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007200, C435S007910, C435S007920

Reexamination Certificate

active

07927819

ABSTRACT:
The present invention relates to a method for stabilization of analytes in solutions of solubilized body samples. The method comprises the steps of solubilizing the body samples obtained from a subject in a suitable sample medium and stabilizing said body sample contained within the sample medium by heating said sample medium for a certain period of time. A further aspect of the invention is a method of a denaturing immunoassay of proteins in solution. The method comprises the steps of bringing a sample containing proteins into contact with a denaturing agent and heating said sample in the presence of the denaturing agent to allow the protein to be denatured.

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Kella N K D et al: “Effect of cetyltrimethylammonium bromide on the heat-induced denaturation and protein-protein interactions of arachin at pH 3.6”, Int. Journal of Peptide and Protein Research, vol. 26, No. 2, 1985, pp. 179-186, XP009069323, *abstract*, *pp. 179-181*.
Kella N K D et al: “Effect of sodium dodecyl sulfate on the heat denaturation and aggregation of arachin at pH 3.6” Int. Journal of Peptide and Protein Research, vol. 25, No. 3, 1985, pp. 308-315, XP009069322, *pp. 308-310*.

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