Method for specific binding assays using a releasable ligand

Details Method for specific binding assays using a releasable ligand Method for specific binding assays using a releasable ligand

1993-02-12

1994-07-26

Saunders, David

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

435 75, 435 792, 435 793, 435 794, 436501, G01N 33543

Patent

active

053326792

ABSTRACT:
Immunoassays and DNA probe assays utilizing a non-immune, reversible binding displacement system are provided. In the assay, a releasable ligand, a binding partner for the releasable ligand, an analyte of interest, an analytically detectable (reporter) group, and at least one binding partner for the analyte, are first attached to an insoluble phase so as to form reporter-labeled complex bound to an insoluble phase, followed by the addition of a displacer ligand which displaces the releasable ligand along with some portion of the reporter-labeled complex, so that the released reporter is analytically detectable in a free liquid medium and can be related to the concentration of analyte in the sample.

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