Method for soybean transformation and regeneration

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

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800250, 800DIG26, A01H 400

Patent

active

055698344

ABSTRACT:
A method for Agrobacterium-mediated transformation and regeneration of soybean is disclosed. The method utilizes a cotyledon explant which is prepared by first removing the hypocotyl and then tearing the two cotyledons apart at the cotyledonary node. The explant may be inoculated with either a smear of the disarmed Agrobacterium vector or of liquid culture of the bacterium.

REFERENCES:
patent: 4769061 (1988-09-01), Comai
patent: 5015580 (1991-05-01), Christou et al.
patent: 5188642 (1993-02-01), Shah et al.

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