Method for soybean transformation and regeneration

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

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4351723, 435419, 435418, 4352522, 435426, 800250, 800DIG26, 800255, A01H 500, A01H 510, A01H 400, C12N 514, C12N 1582

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058248773

ABSTRACT:
A method for Agrobacterium-mediated transformation and regeneration of soybean is disclosed. The method utilizes a cotyledon explant which is prepared by first removing the hypocotyl and then tearing the two cotyledons apart at the cotyledonary node. The explant may be inoculated with either a smear of the disarmed Agrobacterium vector or of liquid culture of the bacterium.

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