Method for sorting and separating living cells

Details Method for sorting and separating living cells Method for sorting and separating living cells

2008-04-08

2008-04-08

Le, Long V. (Department: 1641)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

C435S007200, C435S007720, C435S007900, C435S007910, C435S007920, C435S021000, C435S023000, C435S024000, C435S029000, C435S030000, C435S168000, C435S173200, C435S173400, C435S173900, C435S174000, C435S176000, C435S177000, C435S178000, C435S192000, C435S194000, C435S207000, C435S380000, C435S308100, C435S960000, C435S968000, C436S519000, C436S525000, C436S528000, C436S529000, C436S539000, C436S063000, C436S073000, C436S080000, C436S182000, C436S801000, C436S905000, C210S702000, C210S719000, C210S721000, C210S

Reexamination Certificate

active

10105628

ABSTRACT:
We disclose methods of sorting or separating mixtures of living cells (e.g., eukaryotic, prokaryotic, mammalian, pathogenic, bacterial, viral, etc.). We perform our methods by activating cell-selective photophoric labels, which photosensitize and chemically reduce a photosensitive metal compound to form metal grains, particles or crystals. The metal adheres to the cells and forms the basis for sorting or separating different cell types. Photophoric labels may include chemiluminescent agents such as peroxidase enzymes activated with peroxidase substrates capable of luminescence. Photosensitive metal compounds may be present in a light-sensitive matrix or emulsion containing photosensitizable metal compounds, which form metal grains, particles or crystals upon exposure to a developer solution. Developer solutions are formulated to substantially allow living cells to remain viable after exposure to the developing solution. Our methods are useful in flow cytometry and fluorescence activated cell sorting (FACS) methods, microdissection methods, and for attracting, sedimenting, depositing, layering, attaching, adhering, bonding, binding, trapping or suspending cells on surfaces.

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