Method for solubilizing proteins in organic solvents

Details Method for solubilizing proteins in organic solvents Method for solubilizing proteins in organic solvents

1996-12-03

1998-05-26

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Preparing granular- or free-flowing enzyme composition

530345, 530344, A61K 3800

Patent

active

057563314

ABSTRACT:
The present invention relates to a method for producing a protein composition soluble in organic solvents, comprising mixing a protein of interest with a surfactant and a water immiscible organic solvent in amounts and under conditions conducive to the formation of a reverse micelle solution, and evaporating the resulting reverse micelle solution to dryness.

REFERENCES:
Yoshio Okahata et al., "A Lipid-Coated Lipase As A New Catalyst For Triglyceride Synthesis In Organic Solvents", Journal of Chemical Society, Chemical Communications, No. 20, 15 Oct. 1988, pp. 1392-1394.
Powers et al., "Enhanced Solubility Of Proteins And Peptides In Nonpolar Solvents Through Hydrophobic Ion Pairing", p. 928, col. JH, lines 12-20, paragraph 2.

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