Method for solubilizing microbial protein obtained from Chlamydi

Details Method for solubilizing microbial protein obtained from Chlamydi Method for solubilizing microbial protein obtained from Chlamydi

1984-07-06

1987-05-05

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Lysis of micro-organism

435 7, 435 29, 435804, 435816, 435820, 435822, 436510, 436 17, 436174, 436824, 436825, 436543, G09B 1900, G01N 3353

Patent

active

046632913

ABSTRACT:
A method of specimen treatment preparatory to conducting an immunoassay is disclosed whereby a microbial protein is solubilized by a detergent at elevated temperatures and in the presence of an alkali or alkaline earth metal ion. At elevated temperatures, the detergent is soluble. However, at lower temperatures, the presence of the metal ion renders the detergent insoluble so that it is prevented from interacting in the immunoassay procedure. A specific application is in the solubilization of the principal outer membrane protein of Chlamydia trachomatis.

REFERENCES:
patent: 4206014 (1980-06-01), Reichert et al.
patent: 4497899 (1985-02-01), Armstrong et al.
Helenius, et al, Methods in Enzymology, vol. LVI, (1979), pp. 734-749.

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