Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Chemical modification or the reaction product thereof – e.g.,...
Patent
1991-04-30
1992-09-29
Cashion, Jr., Merrell C.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Chemical modification or the reaction product thereof, e.g.,...
435 701, 530300, 530316, 530345, 530350, 530403, 530404, 530405, 530406, 530412, C07K 300, C07K 302
Patent
active
051515024
ABSTRACT:
A process for solubilizing membrane receptor proteins which includes exposing an outer membrane having the desired protein to an oxidizing agent, treating the membrane with a detergent and treating with an inhibitory material lowering agent. This method maintains the activity of the membrane proteins after solubilization.
REFERENCES:
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Forget, G. and Heisler, S. (1979) Experientia 35, 125-126 "Effect of various solubilizers on angiotensin II receptors in bovine adrenocortical plasma membranes".
Rogulja, I., King, S., Erickson, J., Abhold, R., and Harding, J. W. (1986) J. Hypertension 4, S440-S442, "Solubilized Angiotensin Receptors from Bovine Adrenal Corte: Comparison of 125-I-ANG II and 125-I-Sar(1),11e(8)-ANG II Binding".
Capponi, A. M., Birabeau, M. A., and Vallotton, M. B. (1983) J. Receptor Research 3, 289-299 "Solubilization of Adrenal Angiotensin II Receptors With a Zwitterionic Detergent".
Marie, J., Seyer, R., Lombard, C., Desarnaud, F., Aumelas, A., Jard, S., and Bonnafous, J-C. (1990) Biochemistry 29, 8943-8950 "Affinity Chromatography Purification of Angiotensin II Receptor Using Photoactivable Biotinylated Probes".
Monshizadegan Hossain
Webb Maria L.
Cashion Jr. Merrell C.
E. R. Squibb & Sons Inc.
Park Ellen K.
Rozycki Andrew G.
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