Method for single nucleotide alteration

Details Method for single nucleotide alteration Method for single nucleotide alteration

1980-03-24

1982-09-28

Marantz, Sidney

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 68, 435172, 435317, 536 27, C12N 1500

Patent

active

043519016

ABSTRACT:
A method for altering a single nucleotide at a predetermined position in a gene. The method involves the isolation of a single strand gene fragment extending up to the position before the nucleotide to be altered. A ribonucleotide or a protected deoxyribonucleotide corresponding to the desired altered nucleotide is attached at the end of this fragment. The fragment is then annealed to a complementary template which extends beyond the end of the fragment. The fragment is then extended complementary to the remainder of the template. The resulting partially mismatched double-stranded DNA is used to produce a pure DNA gene containing an altered deoxyribonucleotide at the single desired position.

REFERENCES:
patent: 4264731 (1981-04-01), Shine
patent: 4293652 (1981-10-01), Cohen
A. Razin et al., Proc. Nat'l. Acad. Sci. USA, 75(a), 4268-4270, (Sep. 1978).
C. Hutchison et al., J. Biol. Chem., 764, 6551-6560, (1978).

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