Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters plant part growth
Reexamination Certificate
1998-09-18
2001-04-10
Benzion, Gary (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters plant part growth
C435S069100, C435S320100, C435S468000, C536S023600, C800S278000, C800S298000, C800S317000, C800S323100
Reexamination Certificate
active
06215043
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to a gene encoding a transcription factor capable of altering characters of a plant and its use. More particularly, the present invention relates to the PetSPL2 gene which is a novel gene derived from
Petunia hybrida,
genes related thereto, and the use thereof.
2. Description of the Related Art
In order to clarify regulatory mechanisms controlling the characters of a plant, for example, morphogenesis of a flower, molecular biological and molecular geneticical studies have been conducted using
Arabidopsis thaliana, Antirrhinum majus,
and
Petunia hybrida.
In particular,
Petunia hybrida
is preferably used as a subject of studies for the following reasons: high value as a horticultural plant; the presence of various species; ease of transformation; ease to observe due to its large flower; and accumulation of genetical findings (H. Takatsuji, “
Molecular mechanism for determining a shape of a plant”,
Cell Technology, Plant Cell Technology Series (SHUJUNSHA), pp. 96-106).
Genes which cause mutation have been isolated from mutants in which floral organs of the above-mentioned plant is altered. As a result, it is becoming clear that transcription factors play important roles in differentiation and morphogenesis of a flower. For example, SUPERMAN of
Arabidopsis thaliana
is a transcription factor having a zinc finger motif as a DNA binding domain. It is known that, in SUPERMAN mutant with its gene mutated, number of stamens are remarkably increased, and pistils are defective (
THE IDEN,
April, 1997 (Vol. 51, No. 4), pp. 34-38).
For understanding the mechanism for the control of characters of a plant, it is important to identify a novel transcription factor which is involved in such control. A gene for a transcription factor which controls morphogenesis of a flower may be introduced into a plant by using gene engineering procedure. It is possible to obtain a plant, using gene introduction, having a flower with novel characters which has not been obtained or is not likely to be obtained by a conventional breeding. It is considered that a plant with such novel characters is horticulturally valuable.
SUMMARY OF THE INVENTION
A gene of the present invention has DNA which is selected from a) or b): a) DNA having a nucleotide sequence from the 190th position to the 807th position of a nucleotide sequence represented in SEQ ID NO:1 of the Sequence Listing; or b) DNA which hybridizes to DNA of a) under stringent conditions, and encodes a transcription factor capable of altering characters of a plant.
A gene of the present invention encodes a transcription factor which is selected from i) or ii): i) a transcription factor having an amino acid sequence from the 1st position to the 206th position of an amino acid sequence represented in SEQ ID NO:2; or ii) a transcription factor having an amino acid sequence in which one or more amino acids of i) are subjected to deletion, substitution, or addition, and being capable of altering characters of a plant.
In one embodiment of the present invention, the characters of a plant include one selected from the group consisting of the height of a plant and the length of an internode.
A method for producing a transgenic plant of the present invention includes the steps of: introducing a plant cell with the above-mentioned gene; and regenerating a plant body from the plant cell having the introduced gene.
In one embodiment of the present invention, the plant belongs to dicotyledon.
In another embodiment of the present invention, the plant belongs to Solanaceae.
In another embodiment of the present invention, the plant belongs to Petunia.
In another embodiment of the present invention, the gene is incorporated into a plant expression vector.
A transgenic plant of the present invention is produced by the above-mentioned method.
Thus, the invention described herein makes possible the advantages of (1) providing a gene encoding a transcription factor capable of altering characters of a plant, in particular, a height of a plant and a length of an internode; (2) providing a method for producing a plant with its character altered by introduction of the gene; and (3) providing a transgenic plant.
REFERENCES:
patent: WO 90/12084 (1990-10-01), None
Takatsuji, Hiroshi. “Transcription Factors Controlling Floral Organ Development.”Cell Technology.Plant Technology Series. Shujunsha, Japan, pp. 96-106.
Takatsuji, Hiroshi.The IDEN,vol. 51, No. 4, pp. 34-38 (1997).
Torii, K.U. et al.Plant Cell,vol. 8, pp. 735-746 (1996).
Dehio et al.Plant Mol. Bio.,vol. 23, pp. 1199-1210 (1993).
Sakai, Hajime et al. “Role of Superman in maintainingArabidopsisfloral whorl boundaries.”Nature,vol. 378, pp. 199-203 (1995).
Bancroft, E. et al. (The EU Arabidopsis Genome Project: Mt. Bevan) Analysis of 1.9 Mb of contiguous sequence for chromosome 4 ofArabidopsis thaliana. Nature,vol. 391, pp. 485-488 (1998).
Ken-ichi Kubo, et. al.: “Cys2/His2zinc-finger protein family of petunia: evolution and general mechanism of target-sequence recognition”Nucleic Acids Researchvol. 26, No. 2 pp. (608-615).
Nakagawa Hitoshi
Takatsuji Hiroshi
Benzion Gary
Director General of National Institute of Agrobiological Resourc
Mehta Ashwin D.
Townsend & Townsend & Crew LLP
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