Method for serum-free culture of human vascular endothelial cell

Details Method for serum-free culture of human vascular endothelial cell Method for serum-free culture of human vascular endothelial cell

1993-09-29

1997-11-25

Naff, David M.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Solid support and method of culturing cells on said solid...

435177, 435180, 435366, 435395, 435396, 435404, 435407, C12N 500, C12N 1100, C12N 1102, C12N 1108

Patent

active

056912030

ABSTRACT:
Animal adhesive cells, particularly human vascular endothelial cells, are cultured in serum-free condition by coating at least one polymer having cell adhesive activity on an inner surface of a culture vessel or surface of a carrier for cell culture, and culturing the cells in the coated vessel or with the coated carrier using a serum-free medium for animal cell culture containing isolated serum albumin, and preferably also transferrin. The polymer is a synthetic polymer modified with a peptide having cell adhesive activity or a natural polymer having cell adhesive activity or a combination thereof. Preferably, the peptide is RGDV, RGDS, RGDN, DGEA or YIGSR and the natural polymer is collagen, gelatin, keratin, fibronectin, vitronectin or laminin. A preferred medium for culturing human vascular endothelial cells is basal medium MCDB 131 or MCDB 107 containing isolated serum albumin, transferrin, hydrocortisone and epithelial growth factor.

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Ann Knedler et al., "Optimized Medium for clonal Growth of Human Microvascular Endothelial Cells with Minimal Serum", In Vitro Cellular & Developmental Biology, vol. 23, No. 7, pp. 481-491, (1987).
Hirano et al., Journal of Biomedical Materials Research, vol. 25, 1991, pp. 1523-1534.
Milhausen, S., J. Cell Physiol., vol. 96, 1978, pp. 1-14.

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