Method for sequential determination of an analyte in a fluid sam

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 79, 435 792, 435 794, 435 795, 436512, 436518, 436540, 436543, 436538, C12Q 100, G01N 33535

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active

052348121

ABSTRACT:
The invention relates to an assay for determining an analyte in a fluid sample. Three receptors are used, with formation of a quaternary or four member complex. One of the receptors is bound to a solid phase, and binds to complexes of another receptor and the analyte. A third receptor is labelled, and it, too, binds analyte. The invention also involves the use of a displacement solution to wash fluid sample from solid phase after the first portion of the quaternary complex forms, i.e., prior to binding with the labelled receptor.

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Oellerich, J. Clin. Chem. Biochem. 18: 197-199 (1980).

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