Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Chemical aftertreatment – e.g. – acylation – methylation – etc.
Patent
1997-05-09
1999-10-05
Tsang, Cecilia J.
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
Chemical aftertreatment, e.g., acylation, methylation, etc.
530330, 530344, 530402, 530412, C07K 100, C07K 9900
Patent
active
059626429
ABSTRACT:
A protein or peptide which has an amino-terminus serine or threonine which has an acetylated alpha-amino group is allowed to react with an acid, and then allowed to react with an isothiocyanate under acidic conditions to thereby obtain a thiocarbamyl compound. Then the compound is to be analyzed using Edman degradation. Analysis can be performed with fewer operation steps and without using enzymes.
REFERENCES:
patent: 5468843 (1995-11-01), Boyd et al.
patent: 5534440 (1996-07-01), Aebersold et al.
Ataka Tatsuaki
Sakuhara Toshihiko
Takamoto Keiji
Tsugita Akira
Uchida Toyoaki
Delacroix-Muirheid C.
Seiko Instruments Inc.
Tsang Cecilia J.
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