Method for sequencing of peptides by carboxyl terminus degradati

Details Method for sequencing of peptides by carboxyl terminus degradati Method for sequencing of peptides by carboxyl terminus degradati

1988-04-20

1989-06-06

Hill, Jr., Robert J.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

530345, 530402, G01N 3368

Patent

active

048371653

ABSTRACT:
A peptide is sequenced from its carboxyl terminal by coupling with an isothiocyanate having a chemical formula R(.sub.x)Si(NCS).sub.y where x=0-3 and y=1-4. R may be alkyl or aryl. The coupling reaction is preferably conducted at a temperature range of 50.degree.-90.degree. C. for a period to approximately sixty (60) minutes or less. A weak acid anhydride, preferably a carboxylic acid anhydride, or a weak acid chloride, preferably a carboxylic acid chloride, may be mixed with the isothiocyanate. An organic base catalyst selected from a group consisting of tertiary amines including aromatic amines such as pyridine may also be utilized.

REFERENCES:
patent: 4065412 (1977-12-01), Dreyer
Edman, "Protein Sequence Determination", Edited by S. Needleman, Chapter 8, pp. 211-255, Spring-Verlog, N.Y., 1970.
Stark, Biochemistry, vol. 7, No. 5, pp. 1796-1807, 1968.
Cromwell et al., Biochemistry, vol. 8, No. 12, pp. 4735-4740, 1969.
Meuth et al., Biochemistry, vol. 21, No. 16, pp. 3750-3757, 1982.
Kricheldorf et al., Chemical Abstracts, vol. 79, Abstract No. 53835p, 1973.
Ram et al., Chemical Abstracts, vol. 99, Abstract No. 37638j, 1983.
Ram et al., Chemical Abstracts, vol. 99, Abstract No. 194571z, 1983.
Fokin et al., Chemical Abstracts, vol. 78, Abstract No. 158965p, 1973.
Fokin et al., Chemical Abstracts, vol. 77, Abstract No. 87794y, 1972.
Kodama et al., Chemical Abstracts, vol. 87, Abstract No. 87:68430s, 1976.

Affiliated with

Also associated with

Rating

Method for sequencing of peptides by carboxyl terminus degradati does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for sequencing of peptides by carboxyl terminus degradati, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for sequencing of peptides by carboxyl terminus degradati will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-40371