Method for sequencing nucleic acid sequences without chain...

Details Method for sequencing nucleic acid sequences without chain... Method for sequencing nucleic acid sequences without chain...

2007-09-04

2007-09-04

Chunduru, Suryaprabha (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C536S022100

Reexamination Certificate

active

10344891

ABSTRACT:
A method is provided for determining the sequence of a nucleic acid sequence via incubation of a primer extended product of the nucleic acid sequence in separate pools of semi-random oligonucleotides of 5′ (N)nA3′, 5′ (N)nG3′, and 5′ (N)nT3′, wherein N is A, C, G or T and n is selected so that the oligonucleotides will optimally anneal to all complementary sequences.

REFERENCES:
patent: 5756285 (1998-05-01), Fuller
patent: 5858731 (1999-01-01), Sorge et al.
patent: 5994058 (1999-11-01), Senapathy
Kofoid et al. The 17-gene ethanolamine (eut) operon of salmonella typhimurium encodes five homologues of carboxysome shell proteins. J Bacteriol., vol. 181, No. 17, pp. 5317-5329, 1999.

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