Method for sequencing nucleic acid molecules

Details Method for sequencing nucleic acid molecules Method for sequencing nucleic acid molecules

2004-07-26

2009-10-20

Riley, Jezia (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C435S091200, C536S024300, C536S024330

Reexamination Certificate

active

07604963

ABSTRACT:
The sequence of a target polynucleotide can be determined by: (i) contacting the target polynucleotide with a polymerase enzyme and one of the nucleotides A, T(U), G, and C under conditions suitable for the polymerase reaction to proceed; (ii) measuring the time taken for the polymerase to bind to and subsequently dissociate from the target polynucleotide, to thereby determine whether the polymerase has incorporated the nucleotide onto the target polynucleotide; (iii) optionally repeating steps (i) and (ii) with additional nucleotides, to thereby identify the sequence of the target polynucleotide.

REFERENCES:
patent: 6174670 (2001-01-01), Wittwer et al.
patent: 1245218 (2000-02-01), None
patent: WO 99/05315 (1999-02-01), None
patent: WO 00/53805 (2000-09-01), None
patent: WO 00/70073 (2000-11-01), None
patent: WO 03/027326 (2003-04-01), None

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