Method for sequencing DNA base pairs

Chemistry: analytical and immunological testing – Heterocyclic carbon compound – Hetero-o

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436149, 250340, 250341, G01N 3348, G01N 2518

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active

052702146

ABSTRACT:
The base pairs of a DNA structure are sequenced with the use of a scanning tunneling microscope (STM). The DNA structure is scanned by the STM probe tip, and, as it is being scanned, the DNA structure is separately subjected to a sequence of infrared radiation from four different sources, each source being selected to preferentially excite one of the four different bases in the DNA structure. Each particular base being scanned is subjected to such sequence of infrared radiation from the four different sources as that particular base is being scanned. The DNA structure as a whole is separately imaged for each subjection thereof to radiation from one only of each source.

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Thomas P. Beebe, Jr., "Direct Observation of Native DNA Structures with the Scanning Tunneling Microscope," Science, vol. 243, Jan. 20, 1989, pp. 370-372.

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