Method for separation of .beta.-amylase

Details Method for separation of .beta.-amylase Method for separation of .beta.-amylase

1992-11-10

1994-03-15

Therkorn, Ernest G.

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210656, 127 55, 435 95, 435201, 536103, B01D 1508

Patent

active

052943410

ABSTRACT:
Disclosed is a method of separating .beta.-amylase from a solution containing .beta.-amylase, in which the solution is treated with an .alpha.-cyclodextrin fixed water-insoluble high polymer compound in the presence of ammonium sulfate to form an adsorbed composite of the high polymer compound and .beta.-amylase and soluble impurities not adsorbed to the high polymer compound are separated and removed. By the method, a high-purity .beta.-amylase, which is highly useful in the starch saccharification industry, may specifically and efficiently be isolated from a .beta.-amylase-containing mixture solution.

REFERENCES:
patent: 4539399 (1985-09-01), Armstrong
patent: 4781858 (1988-11-01), Mizukami
patent: 4867884 (1989-09-01), Rendleman
patent: 5080795 (1992-01-01), Pirkle
patent: 5104547 (1992-04-01), Cabrera
patent: 5190663 (1993-03-01), Fetzer
patent: 5204254 (1993-04-01), Schmid

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