Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...
Patent
1994-01-31
1995-07-11
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Maintaining blood or sperm in a physiologically active state...
435 4, 435 725, 435 29, 435 34, 435975, 209199, 2105121, 210782, C12Q 104, C12Q 102, B03D 300
Patent
active
054320542
ABSTRACT:
A method for isolating and enriching rare cells, including fetal nucleated erythrocytes from a peripheral blood sample is described. The method includes two centrifugation steps, the first a bulk separation step to enrich erythrocytes from other blood components. The second centrifugation comprises a colloidal density gradient medium dispersed in a hypertonic meltable gel. In one aspect of the invention, maternal erythrocytes may be hemolyzed prior to the second density gradient centrifugation to provide additional enrichment for fetal nucleated erythrocytes.
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Baldwin Patricia J.
Saunders Alexander M.
Zarowitz Michael A. M.
Applied Imaging
Gromet Nancy J.
Wityshyn Michael G.
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