Method for separating polypeptides

Details Method for separating polypeptides Method for separating polypeptides

1984-10-29

1987-02-24

Kight, John

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Separation or purification

530412, 210672, 210723, 210730, 210732, 210733, 210735, 210905, 210927, A23J 109, B01D 2101

Patent

active

046458298

ABSTRACT:
A method of separating a first polypeptide fraction from a second polypeptide fraction of a mixed solution is disclosed. A predetermined charged polymer is admixed with the mixed solution preferably in the presence of a neutral polymer. The charged polymer interacts with the first fraction to form a precipitate. The neutral polymer enhances the effectiveness of the charged polymer in precipitating the first fraction. By proper selection of the polymer combination the desired polypeptide fraction can be precipitated from solution leaving the other polypeptide fraction in solution or vice versa.

REFERENCES:
patent: 3655509 (1972-04-01), Fields et al.
patent: 3994806 (1976-11-01), Rausch et al.
patent: 4357272 (1982-11-01), Polson
patent: 4518769 (1985-05-01), Wu et al.

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