Method for separating nucleic acids and nucleic acid probes

Details Method for separating nucleic acids and nucleic acid probes Method for separating nucleic acids and nucleic acid probes

1987-11-16

1991-10-22

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, B01D 5702, G01N 2726

Patent

active

050592947

ABSTRACT:
An improved method and device for nucleic acid hybridization assay employing combined direct an alternating field electrophoresis are disclosed. In the method of the present invention, a sample is hybridized with nucleic acid probe and is contacted with a support medium where direct and alternating electric fields are applied. Under the influence of the electric fields, hybrid separates from non-specifically bound nucleic acid probe. The hybrid may be measured on the support medium itself as on a paper strip or in a cartridge containing support medium or may be blotted on an inert surface and then measured. The method an device are useful in the diagnosis of diseases. Kits are provided for assay of a large number of diseases.

REFERENCES:
patent: 4473452 (1984-09-01), Cantor et al.
G. F. Carle and M. V. Olson, "Separation of chromosomal DNA molecules from year by orthogonal-field-alternation gel electrophoresis" Nucleic Acids Research, vol. 12, No. 14 (1984) pp. 5647-5664.

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