Liquid purification or separation – Processes – Chromatography
Patent
1999-08-31
2000-11-21
Therkorn, Ernest G.
Liquid purification or separation
Processes
Chromatography
2101982, B01D 1508
Patent
active
061498181
ABSTRACT:
The object of the present invention is to provide a method for separating components in a liquid specimen and an apparatus used in said method, by which it is possible to avoid carryover even when the specimen injector is not washed using washing solution and also to avoid the increase of inner pressure in column of the separation device or ineffective separating operation even when many liquid specimens are separated by many times using the same separation device. In the present invention, the liquid specimen is introduced into the separation device from a direction opposite to eluting direction of the separation device which retains an adsorbent, and by separating and eluting the specimen from eluting outlet together with the eluant, trace quantity of insoluble substances and the specimen remained on the specimen introducing passage can be removed by elution procedure. As a result, it is possible to avoid carryover, to prevent the increase of inner pressure in the column of the separation device and to prevent ineffective separating operation caused by clogging of the column.
REFERENCES:
patent: 5395521 (1995-03-01), Jagadeeswaran
patent: 5618434 (1997-04-01), Ladisch
patent: 5693223 (1997-12-01), Yamada
Arashima Hideyoshi
Hanafusa Nobuhiro
Hayashi Masayoshi
Nakamura Kenji
Okuyama Tetsushi
Shimadzu Corporation
Therkorn Ernest G.
Wako Pure Chemical Industries Ltd.
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