Imperforate bowl: centrifugal separators – With means for furnishing auxiliary fluid to material or...
Reexamination Certificate
1998-08-13
2001-11-13
Cooley, Charles E. (Department: 1723)
Imperforate bowl: centrifugal separators
With means for furnishing auxiliary fluid to material or...
C494S037000, C494S045000
Reexamination Certificate
active
06315706
ABSTRACT:
AREA OF THE INVENTION
The present invention relates to a method and a bag assembly intended to be used in connection with separation of cells, and specifically for the production of platelets from pooled buffy coats and in other similar areas. The invention also relates to a bag assembly therefor, which is efficient and inexpensive.
PRIOR ART
An apparatus for plasmapheresis is disclosed in EP-BI-0304431, and comprises a round bag and a centrifuge rotor specifically adapted for performing plasmapheresis.
A further improved bag system and centrifuge separator is disclosed in WO 95/01842 and comprises a ring-shaped outer bag and a central inner bag which is formed from the central part of the ring. The outer bag is adapted to form a shortened and conical ring. The inner bag is placed in a rigid centre part which prevents the radial expansion of the inner bag, but allows it to expand axially when filled with liquid. The rigid centre part and the shortened outer ring are placed in a centrifuge rotor.
A container assembly for washing blood cells is disclosed in U.S. Pat. No. 5,114,396, comprising a round bag similar to the bag disclosed in EP-B1-030431. The container assembly comprises, in addition to a ring bag and an inner bag, further bags comprising further liquids used in the washing process. Such further bags are placed in a central rotor compartment on top of the inner bag, and are not used during the centrifugation process. When the centrifugation rotor is stopped, the liquids in said further bags are used for specific purposes.
Normally, when separating platelets from a buffy coat, the separation takes place in a centrifuge in a G-field of suitable size and during a predetermined time duration. Then, the centrifuge is stopped and the bags are removed from the centrifuge and finally extracted in a secondary step through manual proceedings. Such removal and subsequent handling will inevitably cause some mixing and deterioration of the separation. It is necessary to include a cell filter between the centrifuge bag and the storage bag, to inhibit other cells than platelets from being transferred to the storage bag.
DISCLOSURE OF THE INVENTION
The object of the present invention is to suggest a separation process suitable for separation of platelets and similar cells from a suspension and for transportation of the separated cells to a container while maintaining a high purity and at the same time avoid using a cell filter.
A method of separation and a bag assembly for use in such a method are more clearly defined in the appended claims.
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Unger Peter
Westberg Eric
Cooley Charles E.
Gambro Inc.
Jacobson & Holman PLLC
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