Chemistry: analytical and immunological testing – Including sample preparation – Liberation or purification of sample or separation of...
Reexamination Certificate
2008-01-08
2008-01-08
Gabel, Gailene Clare R. (Department: 1641)
Chemistry: analytical and immunological testing
Including sample preparation
Liberation or purification of sample or separation of...
C435S007210, C435S007230, C435S007240, C435S803000, C436S063000, C436S064000, C436S518000, C436S523000, C436S526000, C436S527000, C436S174000, C436S824000, C422S072000, C422S105000
Reexamination Certificate
active
10260560
ABSTRACT:
The present invention is related to a method for separating a first population of cells from a second population of cells in a sample by discontinuous density gradient separation using dense particles to target the first population of cells and a density separation medium (DSM) that is at least about 0.001 g/cm3higher than the density of the second population of cells.
REFERENCES:
patent: 4004975 (1977-01-01), Lionetti et al.
patent: 4111199 (1978-09-01), Djerassi
patent: 4255256 (1981-03-01), Ferrante et al.
patent: 4927749 (1990-05-01), Dorn
patent: 4927750 (1990-05-01), Dorn
patent: 5397479 (1995-03-01), Kass et al.
patent: 5474687 (1995-12-01), Van Vlasselaer
patent: 5482829 (1996-01-01), Kass et al.
patent: 5489386 (1996-02-01), Saunders
patent: 5576185 (1996-11-01), Coulter et al.
patent: 5646004 (1997-07-01), Van Vlasselaer
patent: 5648223 (1997-07-01), Van Vlasselaer
patent: 5676849 (1997-10-01), Sammons et al.
patent: 5739033 (1998-04-01), Soon-Shiong
patent: 5840502 (1998-11-01), Van Vlasselaer
patent: 6004743 (1999-12-01), Kenyon et al.
Patel, D. et al., “Optimization of conditions for specific binding of antibody-coated beads to cells”, Journal of Immunoloogical Methods, 1995, pp. 71-80, vol. 184, Elsevier.
Patel, D. et al., “Use of density perturbation to isolate immunology distinct populations of cells”, Journal of Immunological Methods, 1993, pp. 241-251, vol. 163, Elsevier.
Bildirici, L. et al., “Fractionation of differentiating cells using density perturbation”, Journal of Immunological Methods, 2000, pp. 93-99, vol. 240, Elsevier.
Bildirici, L. et al., “An investigation into the suitability of silica beads for call separations based on density perturbation”, Journal of Immunological Methods, 2001, pp. 57-62, vol. 252, Elsevier.
Patel, D. et al, “Separation of T and B lymphocytes from human peripheral blood mononuclear cells using density perturbation methods”, Clinica Chimica Acta, 1995, pp. 187-193, vol. 240, Elsevier.
Ellis, W.M. et al, “The Use of Discontinuous Percoll Gradients to Separate Populations of Cells from Human Bone Marrow and Peripheral Blood”, Journal of Immunological Methods, 1984, pp. 9-16, vol. 66, Elsevier.
Skoog, W.A. et al., “Studies on the Fibrinogen, Dextran and Phytohemagglutinin Methods of Isolating Leukocytes”, Blood, 1956, pp. 436-454, vol. 11.
Ruijs, W.P.M. et al., “Investigation of the Suitability of a New Purification Medium in Comparison with Percoll™ to Separate Bone Marrow”, 2000, Ishage, Proceedings, p. 24.
Böyum, A., “Isolation of Leucocytes from Human Blood Further Observations”, Scand. J. Clin. Lab. Invest., 1968, pp. 31-50, vol. 21, No. S97.
Böyum, A., “A One-stage Procedure for Isolation of Granulocytes and Lymphocytes from Human Blood”, Scand. J. Clin. Lab. Invest., 1968, pp. 51-76, vol. 21, No. S97.
Lillevang, S.T. et al., “A method for isolating granulocytes from rabbit blood without causing activation”, Journal of Immunological Methods, 1994, pp. 137-138, vol. 169, Elsevier.
Böyum, A., “Isolation of Leucocytes from Human Blood—A two-phase system for removal of red cells with methylcellulose as erythrocyte-aggregating agent”, Scand. J. Clin. Lab. Invest., 1968, pp. 9-29, vol. 21, No. S97.
Fisker, S. et al., “Isolation of rat peritoneal mononuclear and polymorphonuclear leucocytes on discontinuous gradients of Nycodenz”, Journal of Immunological Methods, 1990, pp. 31-38, vol. 133, Elsevier.
Ford, T.C. et al., “A new one-step method for the isolation of human mononuclear cells”, Journal of Immunological Methods, 1990, pp. 237-241, vol. 134, Elsevier.
Noble, P.B. et al., “Ficoll Floatation for the Separation of Blood Leukocyte Types”, Blood, Jan. 1968, pp. 66-73, vol. 31, No. 1.
Ulmer, A.J. et al., “Discontinuous Density Gradient Separation of Human Mononuclear Leucocytes Using Percoll™ as Gradient Medium”, Journal of Immunology Methods, 1979, pp. 1-10, vol. 30, Elsevier.
Pertoft, H. et al., The Viability of Cells Grown of Centrifuged in a new Density Gradient Medium, Percoll™, Experimental Cell Research, 1977, pp. 449-457, vol. 110.
Vaughan, W.P., et al., “Breast Cancer Detected in Cell Culture of Histologically Negative Bone Marrow Predicts Systemic Relapse in Patients with Stage I, II, II and Locally Recurrent Disease”, Proceedings—Am. Soc. Clin. Oncol—26thAnnual Meeting, May 20-22, 1990, p. 9, vol. 9.
Braun, S. et al., “Cytokeratin-Positive Cells in the Bone Marrow and Survival of Patients with Stage I, II, or III Breast Cancer”, The New England Journal of Medicine, Feb. 24, 2000, pp. 525-533, vol. 342, No. 8, Massachusetts Medical Society.
Firat, H. et al., “Comparison of CD34+ bone marrow cell purified by immunomagnetic and immunoadsorption cell separation techniques”, Bone Marrow Transplantation, 1998, pp. 933-938, vol. 21, Stockton Press.
Thomas, T.E. et al, “Positive Selection of CD34+ Cells from Human Bone Marrow for Indirect Purging of Non-Hodgkin's Lymphoma Cells”, Cancer Research, Therapy and Control, 1994, pp. 119-128, vol. 4, No. 2, Harwood Academic Publishers GmbH.
De Wynter, E.A. et al., “Comparison of Purity and Enrichment of CD34+ Cells from Bone Marrow, Umbilical Cord and Peripheral Blood (Primed for Apheresis) Using Five Separation Systems”, Stem Cells, 1995, pp. 524-532, vol. 13.
Shpall, E.J. et al., “Transplantation of Enriched CD34-Positive Autologous Marrow Into Breast Cancer Patients Following High-Dose Chemotherapy: Influence of CD34-Positive Peripheral-Blood Progenitors and Growth Factors on Engraftment”, Journal of Clinical Oncology, Jan. 1994, pp. 28-36, vol. 12, No. 1.
RosetteSep™ Support Reagents, StemCell Technologies 1999/2000 Catalog Supplement, p. 23A.
Bereskin & Parr
Gabel Gailene Clare R.
Gravelle Micheline
Stemcell Technologies Inc.
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