Method for separating and analyzing nucleic acids

Details Method for separating and analyzing nucleic acids Method for separating and analyzing nucleic acids

1984-05-23

1989-01-31

Castel, Benoit

Chemistry: analytical and immunological testing

Heterocyclic carbon compound

Hetero-o

210709, 210745, 436178, 536 27, 536 28, 536 29, G01N 3102, G01N 3350

Patent

active

048015504

ABSTRACT:
The primary and/or secondary structure of nucleic acid as well as its content and molecular weight can be analyzed in solutions by light scatter or fluorescence measurements after treatment with 3 or 4 ring aromatic cations which bind to single-stranded nucleic acids by cooperative association and induce their condensation (collapse). Preparative separation of nucleic acids of different types from the mixtures in the solution is also accomplished by the same principle and techniques wherein each condensed acid is removed from solution and the condensation reversed.

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